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(American Journal of Botany. 1999;86:COVER-cover.)
© 1999 Botanical Society of America, Inc. Cover Illustration: Time series during hydration of an oriental spruce (Picea orientalis) pollen grain. Rhodamine B stains the exine (red) while fluorescein diacetate crosses plasmalemmae and indicates esterase activity in the living cells (green). Swelling of the tube cell reduces the volume of the saccate air space and results in a loss of pollen buoyancy. Confocal extended depth of focus sections taken at (top to bottom) 1, 8, and 15 min after start of hydration. See Runions et al.: Pollination of Picea orientalis(Pinaceae): saccus morphology governs pollen buoyancy, pp. 190–197 in this issue. Photo credit: C. John Runions.
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