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Genetics and Molecular Biology |
Department of Biology, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3 Canada
Received for publication September 5, 2002. Accepted for publication December 5, 2002.
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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Key Words: distyly • heterostyly • immunocytochemistry • polygalacturonase • transmitting tissue • Turnera • Turneraceae
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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). Distylous populations possess two mating types (long- vs. short-styled morphs) that have a reciprocal positioning of male and female reproductive organs (reciprocal herkogamy), the anthers and stigmas. Both morphs commonly possess a self- and intra-morph incompatibility system that enforces inter-morph mating and prevents self-fertilization. Despite a long history of use as a model system in genetics and evolutionary biology (Ornduff, 1992
) beginning with Darwin (1877)
, nothing is known of the molecular genetic basis of the polymorphism including dimorphisms in reproductive organ lengths, self-incompatibility (SI), or ancillary characters.
Genetic studies of distylous species indicate that all dimorphic characters, including the SI system, commonly appear to be determined by a single diallelic locus, where short-styled plants are usually heterozygous, Ss, and long-styled plants are homozygous recessive, ss (Lewis and Jones, 1992
). The dominance relationship is reversed in two families (Baker, 1966
; Ornduff, 1979
). Studies of Primula spp. (Primulaceae) indicate that the distyly locus is actually composed of at least three tightly linked loci that are held in extreme linkage disequilibrium and comprise a supergene (Ernst, 1955
; Dowrick, 1956
; Lewis and Jones, 1992
; Richards, 1997
). In fact, distyly is one of the best examples of a supergene, yet the molecular genetic basis of the system is unknown, although candidate proteins/molecules have been proposed (Golynskaya et al., 1976
; Shivanna et al., 1981
; Wong et al., 1994
; Athanasiou and Shore, 1997
).
Athanasiou and Shore (1997)
discovered proteins specific to the styles and pollen of the short-styled morph of Turnera subulata Smith, T. scabra Millsp., and a few plants of T. krapovickasii Arbo. In the present study, we extend the work of Athanasiou and Shore (1997)
by sequencing, identifying, and localizing the proteins in T. subulata. The style-specific protein is a polygalacturonase (PG) localized to the transmitting tissue of the short-styled morph of T. subulata. Linkage analysis reveals that the style PG gene is linked to and its expression may be upregulated by the S allele of the distyly locus. The pollen-specific protein is also a polygalacturonase.
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MATERIALS AND METHODS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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) and stained with coomassie brilliant blue. The most heavily stained protein band with an isoelectric point (pI) of 6.5 was excised from the gel and digested with Lys-C. The resulting peptides were fractionated by high-performance liquid chromatography. Individual fractions were subjected to Edman degradation and four amino acid sequences were obtained (performed by the Protein Chemistry Core Facility of the Baylor College of Medicine). Using extracts of anthers, an identical protocol was followed for the pollen protein (Athanasiou and Shore, 1997
).
DNA sequencing
Degenerate primers were designed based on partial peptide sequences from the style protein. These primers were used to amplify a genomic DNA sequence using the polymerase chain reaction (PCR). The amplified fragment was sequenced. We designed new primers based upon this initial DNA sequence and used 3' RACE (rapid amplification of cDNA 3' end) to obtain much of the coding sequence (starting with amino acid residue 107). Genomic DNA was extracted according to Doyle and Doyle (1987)
. Total RNA was extracted from styles (Jones et al., 1985
). We used walking PCR (Katz et al., 2000
) to obtain the remainder of 5' end of the sequence from genomic DNA, because 5' RACE failed to yield any new sequence data. Sequencing of both strands was performed on the PCR-amplified DNA using cycle-sequencing on an ABI373A sequencer (Applied Biosystems, Foster City, California, USA) at the York University Molecular Biology Core Facility.
For the pollen protein, we amplified a fragment from genomic DNA using degenerate primers designed from partial peptide sequences. This DNA fragment was used to screen a pollen cDNA library (Athanasiou, 2001
). A single clone was isolated and both strands of the cDNA were sequenced. The sequence data have been submitted to GenBank and accession numbers may be found in the Appendix (http://ajbsupp.botany.org/v90).
The PCR amplifications were generally performed using the following conditions with minor modifications depending upon the primers used: to 1 µL (approximately 50 ng) of genomic DNA was added 40.75 µL nuclease-free water, 1 µL dNTP mix (10 mmol/L each dATP, dCTP, dGTP, dTTP), 1 µL 5' primer (5 pmol/µL), 1 µL 3' primer (5 pmol/mL), 5 µL 10x buffer (100 mmol/L Tris-HCl pH 8.8, 500 mmol/L KCl, 15 mmol/L MgCl2, 1% Triton X-100), and 1.25 units of AmpliTaq DNA polymerase (Perkin Elmer/Applied Biosystems, Foster City, California, USA). The samples were processed in a PE-9600 thermal cycler (Perkin Elmer, Boston, Massachusetts, USA) for 35 cycles at 94°C for 1 min, 55°C for 1 min, 72°C for 2 min, with a final extension at 72°C for 5 min. The PCR products were run on ethidium bromide-stained agarose gels (0.8% in TBE buffer) to verify the specificity of the PCR reaction and for further gel purification and sequencing. Detailed information on PCR, 3' RACE, cloning, library construction, screening, and sequencing protocols are found in Athanasiou (2001)
.
Phylogenetic analysis
Amino acid sequences of both the style and pollen proteins were deduced from the cDNA sequences. The amino acid sequences we obtained were compared to plant PG sequences recently used in the phylogenetic analyses (Hadfield et al., 1998
; Hong and Tucker, 2000
; Torki et al., 2000
; Markovi
and Janeek, 2001
; Appendix at http://ajbsupp.botany.org/v90). Amino acid sequences were aligned using Clustal X (Thompson et al., 1997
). The N-terminal region (corresponding to the first 85 amino acids of the style PG, Fig. 1A) and the last 15 amino acids of the C-terminal region were removed from all sequences because of considerable gap formation. The truncated sequences were then realigned. Gaps in the sequences were coded as unknowns. The gene tree was constructed using ordinary protein parsimony analysis of PHYLIP (Felsenstein, 2001
) and rooted using a fungal outgroup (Aspergillus flavus, Fig. 2, AspflB.pg). The percentage of bootstrap samples in which a particular node occurred was calculated based upon 1000 random samples of the sequences with replacement (Felsenstein, 1985
).
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Three styles from a single flower were ground on ice in 20 µL of phosphate-buffered saline (pH 7.4). Loading buffer (54% glycerol, 8% sodium dodecylsulfate, 25% mercaptoethanol, 0.024% bromophenol blue) was added to each sample, vortexed briefly, heated in a boiling water bath for 3 min, and centrifuged at 13 000 g for 5 min. The extracts and a molecular weight ladder (Kaleidoscope or Precision marker proteins; BIO-RAD, Mississauga, Ontario, Canada), were run using discontinuous (5% stacking, 10% resolving) sodium dodecylsulfate-polyacrylamide gels (Hames and Rickwood, 1987
). Proteins were stacked at 50 V and then resolved at 140 V. Separated proteins were transferred electrophoretically (approximately 16 h at 4°C, 30 V, followed by 1 h at 100 V), in Towbin-buffered saline (25 mmol/L Tris-HCl, 192 mmol/L glycine, 20% methanol, pH 8.3), to 0.2 µm Immuno-blot polyvinylidene difluoride (PVDF) membranes (BIO-RAD), according to manufacturer's instructions.
Crude extracts of pollen, using all anthers from a single flower, were run on IEF gels (Athanasiou and Shore, 1997
). Following electrophoretic separation, the pollen proteins were transferred electrophoretically in 0.7% acetic acid to PVDF membranes (overnight at 30 V).
Immunostaining of proteins transferred to PVDF membranes was according to Riggs and Hasenkampf (1991)
, with minor modification. Membranes were first blocked for 30 min and then incubated with a 1 : 2000 dilution of the primary antibody (2 h) in tris-buffered saline containing 0.05% Tween 20, pH 7.5 (TBST). After three 10-min washes with TBST, the membranes were incubated with a 1 : 10 000 dilution of the secondary antibody (monoclonal goat anti-rabbit antibody conjugated to alkaline phosphatase) in TBST (1 h). Following three additional washes in TBST, the membranes were incubated in 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (NBP-BCIP) at 37°C in the dark. All immunostaining procedures, with the exception of the final colorimetric reaction, were carried out with gentle agitation.
Immunocytochemistry
Styles were vacuum-infiltrated for 45 min and fixed for an additional 4 h in (3 : 1) ethanol : glacial acetic acid. Styles were dehydrated through a graded series of ethanol : tertiary butyl alcohol (TBA) and were finally equilibrated to 100% TBA and infiltrated with Tissueprep wax (Fisher, Nepean, Ontario, Canada) at 62°C. Wax-embedded styles were cross-sectioned to 7-µm thickness. Sections were stored at 4°C for several weeks without any activity loss. Sections were expanded by floating at 37°C in double-distilled water (ddH2O) and placed on Biobond-coated (Cedarlane, Hornby, Ontario, Canada) glass slides. Slides were then placed on a warming tray at 35°C (overnight) to adhere the sections to the slides. Slides were passed through two 15-min changes of histoclear (Sigma, Oakville, Ontario, Canada), followed by washes in a graded series of ethanol and ddH2O ending with ddH2O and a final wash in buffer (Tris-HCl-NaCl, 100 mmol/L Tris, 120 mmol/L NaCl, 30 min). Sections were blocked for 30 min by incubation in 200 µL of blocking solution (normal goat serum diluted 1/20 in wash buffer). Blocking solution was shaken gently from the slides, which were then incubated with primary antibody diluted 1/100 in wash buffer. Sections were washed for 3 x 10 min and then incubated with secondary antibody (CY3-conjugated affinipure goat anti-rabbit IgG, H+L; Jackson ImmunoResearch, West Grove, Pennsylvania, USA), for immunodetection. Sections were washed again, and aqueous mounting medium (ProLong Antifade Kit, Molecular Probes, Eugene, Oregon, USA) was applied. Sections were viewed and photographed using a Lietz Dialux UV fluorescence microscope and appropriate filters (TRITC/Dil filters, exciter: D540/25, emitter: D605/55, beamsplitter: 565dcip; Chroma Technology, Brattleboro, Vermont, USA).
Linkage analysis
We mapped four loci using a test cross between a long- and a short-styled plant. The long-styled plant was from T. subulata. The short-styled plant was of hybrid origin, from a backcross between a short-styled plant of T. subulata and a hybrid plant we had produced from a cross between T. subulata and T. krapovickasii. The hybrid plant carried a somatic mutation that made it self-compatible and phenotypically homostyled, having both long stamens and long styles (F. Tamari and J. S. Shore, unpublished data).
The short-styled plant was heterozygous at four loci including Aconitase-1 (Aco-1, heterozygous for the Aco-1M and Aco-1S alleles), cytosolic 6-phosphogluconate dehydrogenase (Pgd-c, heterozygous for the Pgd-cF and Pgd-cS alleles, Athanasiou and Shore [1997]
), Distyly (SS*, in which S* is the mutant allele conferring the homostyled phenotype in S*S* and S*s genotypes, while the dominant S allele yields the short-styled phenotype; F. Tamari and J. S. Shore [unpublished data]), and two TsPG alleles (of the style PG gene) were identified by segregation of a cleaved amplified polymorphism (CAP marker, described later). The other parental plant was long-styled and homozygous at all four loci (homozygous for the Aco-1F allele, Pgd-cS allele, s at Distyly, and homozygous at TsPG). The distyly locus, Aco-1, and Pgd-c were all known to be linked (Athanasiou and Shore, 1997
).
We amplified a 419-bp region of TsPG from genomic DNA of the parental plants and 169 progeny using the primers 5'-CAGTACTTCCATAGAACCTCAA-3' and 5'-GCCCCTGTTAGTTCCAAGATT-3' to detect alleles of the style PG gene. The 419-base pair (bp) region was gel purified and digested with KpnI and separated on 1.5% agarose gels containing ethidium bromide (0.5 µg/mL). The homozygous long-styled parent possessed a single KpnI restriction site cutting the amplified DNA into two fragments of 92 bp and 327 bp. This allele was derived from T. subulata. The short-styled parent was heterozygous possessing one allele yielding the 92-bp and 327-bp fragments, while the other allele (derived from T. krapovickasii) possessed an additional KpnI restriction site resulting in the cleavage of the 327-bp fragment into two additional fragments of approximately equal size. Recombination frequencies and their standard errors among pairs of loci were determined using maximum-likelihood estimation.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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used nondenaturing isoelectric focusing (IEF) to discover proteins specific to styles or pollen of the short-styled morph of Turnera subulata, T. scabra, and T. krapovickasii. The style PG protein sequence (Fig. 1A) is predicted to have 473 amino acid residues, with a molecular mass of approximately 50.4 kD and a pI of 6.6. Without the 31-amino-acid-predicted signal peptide (Nielsen et al., 1997
), the molecular mass would be 46.9 kD and pI 6.0. The predicted pI is in close correspondence with the pIs of 6.1–6.5 of the style proteins reported in Athanasiou and Shore (1997)
. The identity between the amino acid sequence inferred from cDNA and those based upon the four peptide sequences we obtained indicates that we have amplified and sequenced the gene corresponding to the style protein discovered by Athanasiou and Shore (1997)
. The style amino acid sequence contains four domains highly conserved among plant polygalacturonases (Torki et al., 2000
) and appears most similar to a PG from Glycine max (Mahalingam et al., 1999
) with 59.2% identity at the amino acid level (Fig. 1A).
Remarkably, the short-specific pollen protein also appears to be a polygalacturonase based upon its sequence similarity and possession of conserved amino acid sequences around the putative active site (Torki et al., 2000
; Fig. 1B). The pollen PG has 61% identity to a PG from Salix gigliana (Fig. 1B). The pollen PG has a predicted molecular mass of 40.8 kD and a pI of 6.6 or 6.8 with or without the predicted signal peptide (Nielsen et al., 1997
; Fig. 1B), in agreement with an earlier report (Athanasiou and Shore, 1997
).
Phylogenetic analysis
A gene tree of plant polygalacturonases (Fig. 2) places the style PG (TsPG) in clade E and the pollen PG (TsPP) in clade C, based on previous analyses of plant polygalacturonases (Hadfield et al., 1998
; Hong and Tucker, 2000
; Torki et al., 2000
; Markovi and Janeek, 2001
). The style PG is closely allied with PGs of G. max (Glyma1.pg, Fig. 2; Mahalingam et al., 1999
), Arath11.pg (Fig. 2; Torki et al., 2000
), and Arath12.pg (Fig. 2; Torki et al., 2000
), both of Arabidopsis thaliana (Appendix, http://ajbsupp.botany.org/v90). Interestingly, the style PG does not cluster with any of the pistil-specific PGs of Lycopersicon esculentum (Lyces1.pg, Lyces2.pg, Lyces4.pg Lyces7.pg, Fig. 2, Appendix [http://ajbsupp.botany.org/v90]; Hong and Tucker, 2000
). The pollen PG is in a clade with PGs of dioecious S. gigliana (Salgi2.pp, male flower-specific) and many pollen-specific PGs (Fig. 2, Appendix [http://ajbsupp.botany.org/v90]; Markovi and Janeek, 2001
). Analyses using distance and maximum-likelihood methods also resulted in placement of the style PG and pollen PG in the clades detailed earlier.
Immunoblotting and immunocytochemistry
Immunoblotting of proteins was carried out for both the style and pollen proteins (Fig. 3). Using antibodies against the style PG fusion protein, we revealed a protein of approximately 35 kD in styles of short-styled plants, but not in the long-styled (Fig. 3A–B). We made antibodies to the style PG in two different rabbits and both show that the 35-kD protein is specific to styles of short-styled plants (Fig. 3A–B). One antibody source also shows a 120-kD protein that occurs in both morphs (Fig. 3B). The protein is not morph- or tissue-specific and occurs in all floral and vegetative tissues we have examined (D. Khosravi, F. Tamari, and J. S. Shore, unpublished data). This 120-kD protein does, however, provide a convenient internal marker for protein loading levels.
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).
Immunocytochemical analyses indicate that the style PG is in the transmitting tissue of short styles and in tissues within the stigma of short- but not long-styled plants (Fig. 4). In additional surveys, we have shown that this pattern of staining appears only in styles of short-styled plants for all five species of series Turnera that we have investigated (Khosravi, 2000
). Some staining of vascular tissue is also apparent in the style sections of both morphs (Fig. 4).
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. More recent studies of two species have revealed that 23 of 27 short-styled plants of T. scabra possess the pI 6.8 protein, while none of the 26 long-styled plants possessed it (F. Tamari and J. S. Shore, unpublished data). For T. subulata, all 40 short-styled plants possessed the pI 6.8 protein, while one of 36 long-styled plants showed weak staining of this protein band (F. Tamari and J. S. Shore, unpublished data).
Linkage analysis
A cleaved amplified polymorphism in the style PG gene that was segregating in a test cross we had made was identified. We mapped the style PG gene in this cross relative to the position of the distyly locus and two flanking isozyme loci (Athanasiou and Shore, 1997
). Single locus segregation ratios at all four loci in this cross are comparably distorted (Table 1; F. Tamari and J. S. Shore, unpublished data) and show a considerable excess of progeny carrying alleles derived from the T. subulata recurrent parental species (all ratios are approximately 3.6 : 1). These distorted ratios were common in crosses into the T. subulata genetic background (but not into T. krapovickasii background). Despite these distorted ratios, recombination events occurred on both chromosomes, and the map of Aco-1, Distyly, and Pgd-c is comparable to one obtained previously (Athanasiou and Shore, 1997
). The mapping reveals that the style PG gene, while linked to the distyly locus, is 4.6 cM distal to it, lying outside the Pgd-c marker locus (Fig. 5). This finding indicates that although the style PG gene is linked to the distyly locus, it is perhaps not linked closely enough to be considered a component of a supergene.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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) are divergent polygalacturonases based upon their sequence homology to known polygalacturonases. Determination of their activity in vivo and in vitro using biochemical methods should aid in clarifying their roles in nature. The pollen PG identified may not be involved in distyly. A wider analysis of species within the genus Turnera, series Turnera, led to the finding of short-styled plants of T. krapovickasii, T. joelii, and T. grandiflora that did not possess this specific band of pI 6.8 (Tamari, 2001
). The pollen PG gene might be very closely linked and in extreme linkage disequilibrium with the S allele of the distyly locus in T. scabra and T. subulata. The pollen PG is likely a member of a small multigene family based upon banding patterns on IEF immunoblots having multiple bands, most of which are shared by long-styled plants (Fig. 3C). Small multigene families of highly homologous pollen PGs occur in both monocots and dicots (Brown and Crouch, 1990
; Allen and Lonsdale, 1992
; Hadfield and Bennett, 1998
; Torki et al., 1999
). Furthermore, we note that five amino acid differences in the sequence deduced from sequencing cDNA vs. the peptide sequences occur (Fig. 1B), suggesting that we may have cloned and sequenced a different member of a putative multigene family. The affinity purification of antibodies recognizing specifically the pI 6.8 PG may aid in clarifying the discrepancy among sequences. Currently, we suggest that while the pollen PG is likely involved in the reproductive process (Hadfield and Bennett, 1998
), we do not have sufficient evidence to claim that it plays a role in distyly.
In contrast, the style PG is morph-specific, style-specific, localized to the style transmitting and stigmatic tissues, and appears 24 h prior to anthesis (Athanasiou and Shore, 1997
), coinciding with the time at which an SI system should begin to be functional. Furthermore, the style PG is not possessed by the five self-compatible long-homostyled species we have investigated, including T. aurelii, T. cuneiformis, T. orientalis, T. ulmifolia, and T. velutina (Khosravi, 2000
; Tamari, 2001
). A self-compatible long-homostyle somatic mutant that arose on a branch of an otherwise short-styled plant also lacks the style PG (F. Tamari, D. Khosravi, and J. S. Shore, unpublished data). These features strongly indicate that the style PG plays a role in distyly.
The SDS-PAGE analysis of the style PG revealed a discrepancy between the observed 35-kD molecular mass based upon immunoblotting (Fig. 3A–B) vs. the predicted 46.9-kD molecular mass based upon sequence data (Fig. 1A). This finding indicates that a second peptide, in addition to the signal peptide, may be cleaved from the immature style PG. An acidic prosequence peptide is known to be cleaved from a PG of L. esculentum (DellaPenna and Bennett, 1988
). The occurrence of prosequences has been proposed for Cucumis melo and all members of clade B (Fig. 2; DellaPenna and Bennett, 1988
, Hadfield and Bennett, 1998
). Post-translational processing of the C-terminus is also known to occur for some plant PGs (Hadfield and Bennett, 1998
), which could account for the discrepancy in observed vs. predicted molecular mass. Recently, we have used two-dimensional gel electrophoresis, immunoblotting, and peptide sequencing using a mass spectrometer to provide evidence that a 10-kD protein is homologous to a portion of the N-terminal region of the style polygalaturonse (D. Khosravi, unpublished data). This provides further support for the possibility that a prosequence is cleaved from the immature PG polypeptide.
Interestingly, the style PG appears to belong to a relatively recently identified class of PGs (Markovi and Janeek, 2001
; Fig. 2). The only known sites of expression of these PGs are in Glycine max roots in response to nematode attack (Mahalingam et al., 1999
) and in A. thaliana, in which it is expressed in roots and seedlings (Torki et al., 2000
). The occurrence of this class of PG in style transmitting tissue appears to be novel.
While PGs are involved in cell growth processes (Hadfield and Bennett, 1998
), the style PG is unlikely to determine the length of the short style, but it may play a role in the SI system, given its restriction to the transmitting tissue. That role, however, is uncertain. We suggest that the style PG may operate in at least one of three ways: First, it may operate in an oppositional manner preventing self-pollen tube growth in short-styled plants. The style PG may break down pollen tube walls, leaving open the question of how pollen from long-styled plants remains impervious to this enzymatic activity. In support of this possibility, Tamari et al. (2001)
demonstrated an asymmetry in the lengths of pollen tubes; pollen tubes from short-styled plants were generally inhibited in the stigma. Furthermore, because callose plugs do not form during self-pollination of short-styled plants, these pollen tubes might be inhibited very soon after germination and penetration of the stigma (Tamari et al., 2001
). Second, the style polygalacturonase may operate in a complementary manner, enabling pollen from long-styled plants to grow through the styles of short-styled plants. Under this model, incompatibility of short-styled plants would have to be determined by another, as yet unidentified, protein(s). Finally, oligogalacturonides, which are products of the action of PGs, are known to be active elicitors of plant defenses (Hadfield and Bennett, 1998
; Mahalingam et al., 1999
), leading to the possibility that they may be involved in signalling SI responses of short-styled plants.
Self-incompatibility proteins in flowering plants are diverse and include functionally unrelated proteins (Anderson et al., 1986
; Nasrallah et al., 1987
; Foote et al., 1994
). Polygalacturonases have not been shown to be SI proteins but are known to enhance and suppress rates of pollen tube growth in vitro, depending on their concentration (Roggen and Stanley, 1969
). Proof of the involvement of the style PG in the SI system or in some other aspect of distyly will need to be obtained, perhaps using antisense strategies coupled with functional assays of polygalacturonase activity on pollen tube growth in vitro.
Studies of inheritance and compatibility relationships in the Turnera ulmifolia complex (Shore and Barrett, 1985
; Barrett and Shore, 1987
; Tamari et al., 2001
) are consistent with the possibility that distyly may be determined by a supergene. A direct test of this hypothesis involves the discovery and mapping of the genes determining distyly. Athanasiou and Shore (1997)
found no evidence for recombination between the style PG gene and the distyly locus and estimated that the recombination frequency must be less than 0.87% (0.87 cM). They could not, however, discount the possibility that the style PG protein exhibited morph-limited (short-limited) expression rather than extremely tight linkage. To distinguish between these hypotheses, we have mapped the style PG gene using a cleaved amplified polymorphism (CAP marker), showing that the style PG is 4.6 cM distal to the distyly locus (Fig. 5). This finding indicates that style PG gene is not a component of a supergene but rather its expression is morph-limited and may be upregulated by the dominant S allele of the distyly locus.
With a recombination frequency of 4.6% between the distyly locus and the style PG gene, any initial linkage disequilibrium between the style PG gene and the S allele of distyly would have decayed rapidly. Thus, the complete association between the presence of the style PG in short-styled plants from across a number of Central and South American populations, two ploidy levels and two species, and its absence from long-styled plants (Athanasiou and Shore, 1997
) cannot be explained by linkage disequilbrium. Furthermore, we have shown that this relationship holds in three additional species, T. krapovickasii, T. joelii, and T. grandiflora (Khosravi, 2000
; Tamari, 2001
). Finally, Athanasiou (2001)
produced two long-styled plants that were homozygous recombinants at the Pgd-c marker locus, which implies they were also homozygous for the style PG gene linked to the S allele of distyly. Athanasiou (2001)
did not detect the presence of the style PG protein in these long-styled plants, nor did Athanasiou and Shore (1997)
detect the style PG protein in any of the 13 Pgd-c recombinant long-styled plants, at least half of which should be expected to possess the style PG protein. Thus, we believe this lack of expression of the style PG (derived from short-styled plants) in recombinant long-styled plants supports the possibility that the S allele of distyly upregulates the expression of the style PG gene. If so, this report is the first example of a gene regulated by the distyly locus and indicates that in T. subulata the distyly locus (or supergene) possesses a regulatory capacity.
The occurrence of genes with morph-limited expression is consistent with a model of the evolution of distyly by Lloyd and Webb (1992)
. The population genetic model of Charlesworth and Charlesworth (1979)
is predicated upon the occurrence of a supergene, because of linkage constraints involved in the establishment of the polymorphism. Interestingly, the style PG gene we have mapped is sufficiently closely linked to the distyly locus to have been one of the SI genes initially driven to increase in frequency under the model of Charlesworth and Charlesworth (1979)
. What is unexpected about the gene is its apparent morph-limited expression. A detailed analysis of the molecular genetics of distyly in Turnera spp., as well as in species where distyly has evolved independently, should provide a means of distinguishing among, and/or in refining, these and other evolutionary models.
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FOOTNOTES |
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2 Current address: Center for the Biology of Natural Systems, Queens College of the City University of New York, Flushing, New York 11367 USA
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LITERATURE CITED |
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TOPABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONLITERATURE CITED |
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Anderson M. A. et al 1986 Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata. Nature 321: 38-44[CrossRef]
Arroyo J. S. C. H. Barrett 2000 Discovery of distyly in Narcissus (Amaryllidaceae). American Journal of Botany 87: 748-751
Athanasiou A. 2001 The inheritance and molecular characterization of morph-specific proteins in distylous Turnera. Ph.D. dissertation, York University, Toronto, Ontario, Canada
Athanasiou A. J. S. Shore 1997 Morph-specific proteins in pollen and styles of distylous Turnera (Turneraceae). Genetics 146: 669-679[Abstract]
Baker H. G. 1966 The evolution, functioning and breakdown of heteromorphic incompatibility systems. I. The Plumbaginaceae. Evolution 20: 349-368[CrossRef][Web of Science]
Barrett S. C. H. J. S. Shore 1987 Variation and evolution of breeding systems in the Turnera ulmifolia L. complex (Turneraceae). Evolution 41: 340-354[CrossRef][Web of Science]
Brown S. M. M. L. Crouch 1990 Characterization of a gene family abundantly expressed in Oenothera organensis pollen that shows sequence similarity to polygalacturonase. Plant Cell 2: 263-274
Charlesworth B. D. Charlesworth 1979 A model for the evolution of distyly. American Naturalist 114: 467-498[CrossRef][Web of Science]
Darwin C. D. 1877 The different forms of flowers on plants of the same species. John Murray, London, UK
Dellapenna D. A. B. Bennett 1988 In vitro synthesis and processing of tomato fruit polygalacturonase. Plant Physiology 86: 1057-1063
Dowrick V. P. J. 1956 Heterostyly and homostyly in Primula obconica. Heredity 10: 219-236
Doyle J. J. J. L. Doyle 1987 A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15
Ernst A. 1955 Self-fertility in monomorphic Primulas. Genetica 27: 91-148
Felsenstein J. 1985 Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783-791[CrossRef][Web of Science]
Felsenstein J. 2001 PHYLIP: phylogeny inference package. Version 3.6a. Department of Genetics, University of Washington, Seattle, Washington, USA
Foote H. C. C. J. P. Ride V. E. Franklin-Tong E. A. Walker M. J. Lawrence F. C. H. Franklin 1994 Cloning and expression of a distinctive class of self-incompatibility (S) gene from Papaver rhoeas L. Proceedings of the National Academy of Science USA 91: 2265-2269
Golynskaya E. L. N. V. Bashnikova N. N. Tomchuk 1976 Phytohaemagglutinins of the pistil in Primula as possible proteins of generative incompatibility. Soviet Plant Physiology 23: 169-176
Hadfield K. A. A. B. Bennett 1998 Polygalacturonases: many genes in search of a function. Plant Physiology 117: 337-343
Hadfield K. A. J. K. C. Rose D. S. Yaver R. M. Berka A. B. Bennett 1998 Polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly. Plant Physiology 117: 363-373
Hames B. D. D. Rickwood 1987 Gel electrophoresis of proteins: a practical approach. IRL Press, Washington, D.C., USA
Hong S.-B. M. L. Tucker 2000 Molecular characterization of a tomato polygalacturonase gene abundantly expressed in the upper third of pistils from opened and unopened flowers. Plant Cell Reports 19: 680-683[CrossRef][Web of Science]
Jones J. D. G. P. Dunsmuir J. Bedbrook 1985 High-level expression of introduced chimaeric genes in regenerated transformed plants. EMBO Journal 4: 2411-2418[Web of Science][Medline]
Katz L. E. A. Curtis M. Pfunder L. F. Landweber 2000 Characterization of novel sequences from distantly related taxa by walking PCR. Molecular Phylogenetics and Evolution 14: 318-321[CrossRef][Web of Science][Medline]
Khosravi D. 2000 Putative incompatibility proteins in distylous Turnera species: immunoblot and immunocytochemistry analyses. M.S. thesis, York University, Toronto, Ontario, Canada
Lewis D. D. A. Jones 1992 The genetics of heterostyly. In S. C. H. Barrett [ed.], Evolution and function of heterostyly, 129–150. Springer-Verlag, New York, New York, USA
Lloyd D. G. C. J. Webb 1992 The evolution of heterostyly. In S. C. H. Barrett [ed.], Evolution and function of heterostyly, 151–178. Springer-Verlag, New York, New York, USA
Mahalingam R. G. Wang H. Knap 1999 Polygalacturonase and polygalacturonase inhibitor protein: gene isolation and transcription in Glycine max–Heterodera glycines interactions. Molecular Plant–Microbe Interactions 12: 490-498[CrossRef]
Markovi O. 
. Janeek 2001 Pectin degrading glycoside hydrolases of family 28: sequence-structural features, specificities and evolution. Protein Engineering 14: 615-631
Nasrallah J. B. T.-H. Kao C.-H. Chen M. L. Goldberg M. E. Nasrallah 1987 Amino-acid sequence of glycoproteins encoded by three alleles of the S locus of Brassica oleracea. Nature 326: 617-619[CrossRef]
Nielsen H. J. Engelbrecht S. Brunak G. Von Heijne 1997 Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering 10: 1-6
Ornduff R. O. 1979 The genetics of heterostyly in Hypericum aegypticum. Heredity 42: 271-272[Web of Science]
Ornduff R. O. 1992 Historical perspectives on heterostyly. In S. C. H. Barrett [ed.], Evolution and function of heterostyly, 31–39. Springer-Verlag, New York, New York, USA
Richards A. J. 1997 Plant breeding systems, 2nd ed. Chapman & Hall, London, UK
Riggs C. D. C. A. Hasenkampf 1991 Antibodies directed against a meiosis-specific, chromatin-associated protein identify conserved meiotic epitopes. Chromosoma 101: 92-98[CrossRef][Web of Science][Medline]
Roggen H. P. J. R. R. G. Stanley 1969 Cell-wall-hydrolysing enzymes in wall formation as measured by pollen-tube extension. Planta 84: 295-303[CrossRef][Web of Science]
Shivanna K. R. J. Heslop-Harrison Y. Heslop-Harrison 1981 Heterostyly in Primula. 2. Sites of pollen inhibition, and effects of pistil constituents on compatible and incompatible pollen-tube growth. Protoplasma 107: 319-337[CrossRef][Web of Science]
Shore J. S. S. C. H. Barrett 1985 The genetics of distyly and homostyly in Turnera ulmifolia L. (Turneraceae). Heredity 55: 167-174[Web of Science]
Tamari F. 2001 Investigations of self-incompatibility in distylous and homostylous Turnera and Piriqueta (Turneraceae): pollen tube and immunoblot analyses. M.S. thesis, York University, Toronto, Ontario, Canada
Tamari F. A. Athanasiou J. S. Shore 2001 Pollen tube growth and inhibition in distylous and homostylous Turnera and Piriqueta (Turneraceae). Canadian Journal of Botany 79: 578-591[CrossRef]
Thompson J. D. T. J. Gibson F. Plewniak F. Jeanmougin D. G. Higgins 1997 The CLUSTAL X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25: 4876-4882
Torki M. P. Mandaron R. Mache D. Falconet 2000 Characterization of a ubiquitous expressed gene family encoding polygalacturonase in Arabidopsis thaliana. Gene 242: 427-436[CrossRef][Web of Science][Medline]
Torki M. P. Mandaron F. Thomas F. Quigley R. Mache D. Falconet 1999 Differential expression of a polygalacturonase gene family in Arabidopsis thaliana. Molecular and General Genetics 261: 948-952
Wong K. C. M. Watanabe K. Hinata 1994 Protein profiles in pin and thrum floral organs of distylous Averrhoa carambola L. Sexual Plant Reproduction 7: 107-115
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