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First published online December 18, 2009; doi:10.3732/ajb.0900192
American Journal of Botany 97: 156-173 (2010)
© 2010 Botanical Society of America, Inc.
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Systematics and Phytogeography

Molecular systematics of the neotropical genus Psiguria (Cucurbitaceae): Implications for phylogeny and species identification1

P. Roxanne Steele2,3,4, Lauren M. Friar3, Lawrence E. Gilbert2 and Robert K. Jansen2,3

2 Section of Integrative Biology, The University of Texas at Austin, 1 University Station C0930, Austin, Texas 78712 USA 3 Institute of Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station C0930, Austin, Texas 78712 USA

ABSTRACT

Varying morphological features in many groups of tropical vines confound identification, requiring molecular tools for distinguishing species. Confusion is amplified in Psiguria, a small genus found in Central and South America and the Caribbean, because male and female flowers of these monoecious plants are widely separated by time and position on a branch. We present the first phylogeny of Psiguria utilizing a combination of eight chloroplast intergenic spacers, the internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA repeat, and the intron of the low-copy nuclear gene serine/threonine phosphatase, for a total aligned length of 9456 base pairs. Analyses include multiple accessions of all species in the genus. The data support the monophyly of Psiguria and elucidate several species boundaries. Also presented are Psiguria-specific DNA barcodes, which include the chloroplast regions: ndhC-trnV, rps16-trnQ, rpoB-trnC, ndhF-rpl32, and psbZ-trnM. For the first time, systematists, ecologists, and evolutionary biologists will have the tools to confidently identify species of Psiguria with DNA barcodes that may be useful in other genera of Cucurbitaceae.

Key Words: angiosperms • Caribbean • chloroplast • DNA barcoding • Gurania • Guraniinae • Helmontia • ITS • low-copy nuclear • phylogenetics

Received for publication 2 July 2009. Accepted for publication 1 October 2009.

FOOTNOTES

1 The authors thank B. B. Simpson, D. R. Hansen, C. R. Linder, and two anonymous reviewers for valuable comments on the manuscript. The authors acknowledge the following research grants: NSF Doctoral Dissertation Improvement Grant DEB 0808294, the Plant Biology Program at The University of Texas at Austin, the American Society of Plant Biology, and the Botanical Society of America. They also thank MO, NY, F, GH, and TEX herbaria for approving the use of leaf material on loans for DNA studies; M. Condon (Cornell College), S. Swensen (Ithaca College), R. Abbott (University of Florida), T. S. Quedensley (University of Texas at Austin), and H. Schaefer (University of Munich) for providing leaf material; R. Fernández and P. Protti (Costa Rica), F. Axelrod (University of Puerto Rico-Río Piedras), T. Clase (Jardín Botánico Nacional Dominican Republic), O. Plata (Herbario Nacional de Bolivia), and K. Meza (Perú) for field assistance and collecting plants; M. Timaná (Pontificia Universidad Católica del Perú) for help with plant-collecting permits; and finally UT Austin’s Brackenridge Field Laboratory for providing facilities and staff assistance that maintained living collections used in the work.

4 Author for correspondence (e-mail: roxisteele{at}msn.com); present address: University of Missouri-Columbia, 1201 Rollins St.-Bond LSC 311, Columbia, Missouri 65211 USA


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