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(American Journal of Botany. 2008;95:577-587.) doi: 10.3732/ajb.2007320 © 2008 Botanical Society of America, Inc.

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Genetic structure and diversity in Ramonda myconi (Gesneriaceae): effects of historical climate change on a preglacial relict species¹

CREAF (Center for Ecological Research and Forestry Applications), Autonomous University of Barcelona, E-08193 Bellaterra, Spain

ABSTRACT

The importance of the Mediterranean Basin as a long-term reservoir of biological diversity has been widely recognized, although much less effort has been devoted to understanding processes that allow species to persist in this area. Ramonda myconi (Gesneriaceae) is a Tertiary relict plant species restricted to the NE Iberian Peninsula. We used RAPD and chloroplast markers to assess the patterns of genetic structure in eight mountain regions covering almost the full species range, to identify the main historical processes that have shaped its current distribution and to infer the number and location of putative glacial refugia. While no cpDNA variation was detected, the species had relatively high levels of RAPD variation. Maximum levels of diversity were found within populations (71%), but there was also a significant differentiation between geographical regions (20%) and among populations within regions (9%). A spatial AMOVA identified three main groups of populations, corresponding to previously recognized centers of endemism and species richness. In addition, we found a marked geographical pattern of decreasing genetic diversity and increasing population differentiation from west to east. Our results support a complex phylogeographic scenario in the Iberian Peninsula of "refugia-within-refugia" and suggest that the higher diversity observed in western regions might be associated with prolonged and more stable climatic conditions in this area during the Quaternary.

Key Words: chloroplast markers • fragmentation • Gesneriaceae • glacial refugia • Mediterranean climate • NE Iberian Peninsula • paleoendemic species • Quaternary climatic changes • Ramonda myconi • RAPD

Received for publication 4 October 2007. Accepted for publication 27 February 2008.

FOOTNOTES

¹ The authors gratefully acknowledge S.C. González-Martínez, J.A. Rosselló, Ll. Sáez, and V. Rull for constructively criticizing the manuscript. They also thank J. Terradas for financial support, M. Pitet for laboratory assistance, and X. Domene for field work. This research was supported by funds from Institut d’Estudis Catalans (project number LF22I003) and by a grant (2004FI01255) from DURSI (Departament d’Universitats, Recerca i Societat de la Informació, Generalitat de Catalunya) to M. Dubreuil.

² Author for correspondence (e-mail: Maria.Mayol{at}uab.es)

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