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Cryptic species and host specificity in the ectomycorrhizal genus Strobilomyces (Strobilomycetaceae)¹

Laboratory of Plant Taxonomy and Evolution, Department of Botany, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan; Research Institute of Humanity and Nature, National Institutes for the Humanities, Inter-university Research Institute Corp., Kyoto 603-8047, Japan; Makino Herbarium, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan

ABSTRACT

Taxonomical classification of higher fungi remains an important challenge and can benefit from the application of molecular analysis. We propose that the ectomycorrhizal (EM) fungal taxa might include a number of cryptic species because there are few morphological characteristics useful for distinguishing among these fungi. Previously, host specificity in most EM fungi was thought to be low, but we suspect that confusion of cryptic species has led to an underestimate of fungal host specificity. We analyzed both nuclear and mitochondrial DNA sequences from Strobilomyces fungi and obtained evidence that what were previously described as four species can be grouped into 14 distinct lineages, suggesting that these lineages might be distinct biological species. Moreover, we identified host plants for Strobilomyces via nucleotide sequencing of both fungal and plant DNA from EM samples. Most lineages of Strobilomyces tested in this study were associated only with Fagaceae trees, even though Strobilomyces species were previously thought to be generalists with regard to hosts. Thus, we present an approach useful for identifying cryptic species and detecting the true host range of a set of EM fungi in natural conditions.

Key Words: atp6 • cytonuclear system • host range • mutualism • RPB1