HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Archibald, J. K. Articles by Mort, M. E. Search for Related Content PubMed Articles by Archibald, J. K. Articles by Mort, M. E. Agricola Articles by Archibald, J. K. Articles by Mort, M. E.

The utility of automated analysis of inter-simple sequence repeat (ISSR) loci for resolving relationships in the Canary Island species of Tolpis (Asteraceae)¹

²Department of Ecology and Evolutionary Biology and the Natural History Museum & Biodiversity Research Center, University of Kansas, Lawrence, Kansas 66045 USA; ³Jardín de Aclimatación de la Orotava (ICIA), Puerto de la Cruz, Tenerife, Canary Islands, Spain

ABSTRACT

Plants of oceanic islands, often remarkably divergent morphologically from continental relatives, are useful models for studying evolution and speciation because evolution is telescoped in time and space. Prior studies revealed little DNA sequence variation within the clade of ca. 10 Canary Island species of Tolpis, which precluded resolving species relationships. The present study assessed the utility of automated analysis of inter-simple sequence repeat (ISSR) loci for resolving relationships within the clade using 264 individuals from 36 populations of all recognized species and three undescribed morphological variants. Similarity (Dice coefficient) and Fitch parsimony were used to generate neighbor-joining (NJ) and strict consensus trees (MP), respectively. All individuals of the morphologically distinct endemic species formed clusters in both trees. There is also support for clusters of two undescribed variants in the NJ tree. Individuals from a morphologically variable complex consisting primarily of two species are not well resolved at population or species levels. The NJ and MP trees are not congruent at deeper levels, including relationships among species. Results are interpreted in terms of the biology of the species, and the utility of automated analysis of ISSR markers for interpreting patterns of evolution of Tolpis in the Canary Islands is discussed.

Key Words: Asteraceae • Canary Islands • Cichorieae • inter-simple sequence repeat • ISSR • Macaronesia • Tolpis

This article has been cited by other articles:

				M. E. Mort, J. K. Archibald, C. P. Randle, N. D. Levsen, T. R. O'Leary, K. Topalov, C. M. Wiegand, and D. J. Crawford Inferring phylogeny at low taxonomic levels: utility of rapidly evolving cpDNA and nuclear ITS loci Am. J. Botany, February 1, 2007; 94(2): 173 - 183. [Abstract] [Full Text] [PDF]