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(American Journal of Botany. 2005;92:1425-1431.)
© 2005 Botanical Society of America, Inc.

Anatomy and Morphology

Leaf architecture and direction of incident light influence mesophyll fluorescence profiles1

Daniel M. Johnson2, William K. Smith2,4, Thomas C. Vogelmann3 and Craig R. Brodersen3

2Department of Biology, Wake Forest University, Winston-Salem, North Carolina 27109-7325 USA; 3Department of Botany and Agricultural Biochemistry, University of Vermont, Burlington, Vermont 05405-0106 USA

ABSTRACT

Light propagation and distribution inside leaves have been recognized as important processes influencing photosynthesis. Monochromatic light absorption across the mesophyll was measured using chlorophyll fluorescence generated from illumination of the cut edge (epi-illumination), as well as the adaxial or abaxial surfaces of the leaf. Species were selected that had basic leaf types: laminar leaf with adaxial palisade layer (Rhododendron catawbiense), needle with palisade (Abies fraseri), and needle without palisade (Picea rubens). Fluorescence was more evenly distributed across the mesophyll for adaxially illuminated leaves with a palisade cell layer, as well as for the needles (cylindrical) without palisade, when compared to fluorescence generated by abaxial illumination. Moreover, fluorescence from green light illumination remained high across the mesophyll of adaxially illuminated R. catawbiense, indicating a possible influence of mesophyll structure on internal light distribution beyond that of chlorophyll levels. These data support the idea that light propagation within the mesophyll is associated with asymmetric mesophyll structure, in particular the presence of palisade cell layers. In addition, we propose that the evolution of a more cylindrical leaf form, such as found in conifer species, may be a structural solution to excessive sunlight that replaces the highly differentiated mesophyll found in most laminar-leaved species.

Key Words: Abies fraseri • chlorophyll • Ericaceae • high-altitude species • leaf orientation • photosynthesis • Picea rubens • Pinaceae • Rhododendron catawbiense






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