HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Syring, J. Articles by Liston, A. Search for Related Content PubMed Articles by Syring, J. Articles by Liston, A. Agricola Articles by Syring, J. Articles by Liston, A.

Evolutionary relationships among Pinus (Pinaceae) subsections inferred from multiple low-copy nuclear loci¹

²Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331 USA; ³Pacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331 USA

ABSTRACT

Sequence data from nrITS and cpDNA have failed to fully resolve phylogenetic relationships among Pinus species. Four low-copy nuclear genes, developed from the screening of 73 mapped conifer anchor loci, were sequenced from 12 species representing all subsections. Individual loci do not uniformly support either the nrITS or cpDNA hypotheses and in some cases produce unique topologies. Combined analysis of low-copy nuclear loci produces a well-supported subsectional topology of two subgenera, each divided into two sections, congruent with prior hypotheses of deep divergence in Pinus. The placements of P. nelsonii, P. krempfii, and P. contorta have been of continued systematic interest. Results strongly support the placement of P. nelsonii as sister to the remaining members of sect. Parrya, suggest a moderately well-supported and consistent position of P. krempfii as sister to the remaining members of sect. Quinquefoliae, and are ambiguous about the placement of P. contorta. A successful phylogenetic strategy in Pinus will require many low-copy nuclear loci that include a high proportion of silent sites and derive from independent linkage groups. The locus screening and evaluation strategy presented here can be broadly applied to facilitate the development of phylogenetic markers from the increasing number of available genomic resources.

Key Words: incongruence • nuclear genes • phylogeny • Pinaceae • Pinus

This article has been cited by other articles:

				K. Watanabe, T. Ohi-Toma, and J. Murata Multiple hybridization in the Aristolochia kaempferi group (Aristolochiaceae): evidence from reproductive isolation and molecular phylogeny Am. J. Botany, July 1, 2008; 95(7): 885 - 896. [Abstract] [Full Text] [PDF]

				M. E. Mort, J. K. Archibald, C. P. Randle, N. D. Levsen, T. R. O'Leary, K. Topalov, C. M. Wiegand, and D. J. Crawford Inferring phylogeny at low taxonomic levels: utility of rapidly evolving cpDNA and nuclear ITS loci Am. J. Botany, February 1, 2007; 94(2): 173 - 183. [Abstract] [Full Text] [PDF]

				A. Willyard, J. Syring, D. S. Gernandt, A. Liston, and R. Cronn Fossil Calibration of Molecular Divergence Infers a Moderate Mutation Rate and Recent Radiations for Pinus Mol. Biol. Evol., January 1, 2007; 24(1): 90 - 101. [Abstract] [Full Text] [PDF]