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Comparative anatomy and morphology of Vitis vinifera (Vitaceae) somatic embryos from solid- and liquid-culture-derived proembryogenic masses¹

Mid-Florida Research and Education Center, Institute of Food Agricultural Sciences, University of Florida, 2725 Binion Road, Apopka, Florida 32703-8504 USA

Ontogeny of somatic embryos of grapevine (Vitis vinifera) produced from solid- and liquid-culture-derived proembryogenic masses (PEM) was compared using light and scanning electron microscopy. Somatic embryos produced from solid-medium-derived PEM (SPEM) had large cotyledons, little or no visible suspensor structure, and a relatively undeveloped concave shoot apical meristem, whereas those from liquid-medium-derived PEM (LPEM) had smaller cotyledons, a distinct suspensor, and a flat-to-convex shoot apical meristem. The convex shoot apical meristem in LPEM-derived somatic embryos formed as early as the heart stage of development; it was 4–6 cell layers deep and rich in protein. Suspensors persisted in fully developed and mature LPEM-derived somatic embryos. The SPEM-derived somatic embryos exhibited dormancy, as do mature zygotic embryos, which also have a rudimentary suspensor, whereas LPEM-derived embryos were not dormant. We hypothesize that the presence of a persistent suspensor in LPEM-derived somatic embryos modulates development, ultimately resulting in rapid germination and a high plant-regeneration rate.

Key Words: cell culture • embryogenesis • grapevine • somatic embryogenesis • Vitaceae • Vitis vinifera

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				S. A. Dhekney, Z. T. Li, T. W. Zimmerman, and D. J. Gray Factors Influencing Genetic Transformation and Plant Regeneration of Vitis Am. J. Enol. Vitic., September 1, 2009; 60(3): 285 - 292. [Abstract] [Full Text] [PDF]