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(American Journal of Botany. 2003;90:526-531.)
© 2003 Botanical Society of America, Inc.

Genetics and Molecular Biology

The ribosomal small-subunit protein S28 gene from Helianthus annuus (Asteraceae) is down-regulated in response to drought, high salinity, and abscisic acid1

Xianan Liu2,3 and W. Vance Baird4,5

2Department of Horticulture and Genetics Graduate Program, Clemson University, Clemson, South Carolina 29634-0375 USA; 3Department of Plant Biology, 190 ERML, University of Illinois, Urbana, Illinois 61801 USA; 4Department of Horticulture, D-136 Poole Agriculture Center, Box 340375, Clemson University, Clemson, South Carolina 29634-0375 USA

A partial cDNA for the ribosomal S28 gene from sunflower was initially cloned and identified to be down-regulated by high salinity, using differential display reverse transcriptase-polymerase chain reaction (PCR). Using this sequence, a 502-base pair (bp) full-length cDNA was cloned by rapid amplification of cDNA ends. This cDNA (designated Ha-RPS28) encodes a protein component of the small subunit of cytoplasmic ribosomes. The predicted 65 amino acid residue sequence of Ha-RPS28, with an estimated molecular mass of 7.5 kD, has 92, 89, and 86% identity with the S28 ribosomal proteins from peach, maize, and Arabidopsis, respectively. Ha-RPS28 was expressed in all organs examined, and the highest level was detected in fully expanded leaves. Furthermore, expression of Ha-RPS28 was down-regulated in both seedling roots and shoots in response to drought, high salinity, or abscisic acid.

Key Words: abiotic stress • Asteraceae; cDNA cloning • environmental stress • gene expression • gene regulation • ribosomal protein • sunflower






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