Am. J. Bot. Plant Physiology
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(American Journal of Botany. 2002;89:248-252.)
© 2002 Botanical Society of America, Inc.

Reproductive Biology

Collection and storage of pollen from Salix (Salicaceae)1

Richard F. Kopp2,5, Charles A. Maynard3, Patricia Rocha de Niella4, Lawrence B. Smart2 and Lawrence P. Abrahamson2,3

2State University of New York College of Environmental Science and Forestry, Faculty of Environmental and Forest Biology, Syracuse, New York 13210 USA; 3State University of New York College of Environmental Science and Forestry, Faculty of Forestry, Syracuse, New York 13210 USA; 4Misiones National University, School of Forestry Science, Eldorado-Misiones, Argentina

Genetic improvement of willows through traditional breeding can be facilitated by pollen collection and storage so that female flower receptivity need not be synchronized with pollen shed for breeding. Two experiments were completed to test the effectiveness of various organic solvents for willow pollen collection. In the first experiment, seven pollen collection treatments and an untreated control were tested with two willow clones. The other experiment tested three treatments that showed promise in the initial experiment and an untreated control with eight willow clones. Toluene and carbon tetrachloride were effective for pollen extraction, with average pollen germination percentages that were >15%, but both chemicals reduced pollen viability by 10–20% compared with an untreated control based on in vitro germination tests. Pollen extracted with carbon tetrachloride or toluene was successfully used in controlled pollination, and >100 new families were produced with this technique. Pollen viability remained high after 18 mo of storage at –20°C. Based on our results, toluene is the preferred solvent for future willow pollen extractions because it is as effective as carbon tetrachloride, is not a known carcinogen, and is less expensive.

Key Words: breeding • germination • pollination • Salicaceae • Salix • toluene • willow






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