HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Caraway, V. Articles by Morden, C. W. Search for Related Content PubMed Articles by Caraway, V. Articles by Morden, C. W. Agricola Articles by Caraway, V. Articles by Morden, C. W.

Assessment of hybridization and introgression in lava-colonizing Hawaiian Dubautia (Asteraceae: Madiinae) using RAPD markers¹

²Department of Botany, 3190 Maile Way, University of Hawai‘i, Honolulu, Hawai‘i 96822 USA ³Center for Conservation Research and Training, 3190 Maile Way, University of Hawai‘i, Honolulu, Hawai‘i 96822 USA

Hybridization between Dubautia ciliolata and D. scabra occurring on a mosaic of lava flows of 1855 and 1935 on the island of Hawai‘i was examined using random amplified polymorphic DNA (RAPD) markers. The RAPD data indicate that D. ciliolata plants, nearly restricted to the 1855 lava flow, contain higher levels of genetic variation than do D. scabra plants occurring on the 1935 lava flow. Seventy-one markers were specific to D. ciliolata and 60 to D. scabra; 40 of these were "constant" (found in all individuals) in one or the other species. Hybrids sampled were determined to represent F₁, filial hybrids beyond the F₁, and backcross progeny. All backcrosses were unidirectional with D. ciliolata acting as the recurrent parent. No hybrid, including an artificially produced F₁, had all 40 constant markers, suggesting that at least some loci for these markers were heterozygous in the parents. However, several hybrids exhibited a loss of many of the species markers, suggesting that they were later filial hybrid generation plants. The apparent occurrence of unidirectional introgression at the study site may be providing D. ciliolata plants with genetic plasticity to colonize the new lava flow previously occupied only by D. scabra.

Key Words: Dubautia • Hawai‘i • hybridization • introgression • RAPD • silversword alliance

This article has been cited by other articles:

				B. VAN DROOGENBROECK, T. KYNDT, E. ROMEIJN-PEETERS, W. VAN THUYNE, P. GOETGHEBEUR, J. P. ROMERO-MOTOCHI, and G. GHEYSEN Evidence of Natural Hybridization and Introgression between Vasconcellea Species (Caricaceae) from Southern Ecuador Revealed by Chloroplast, Mitochondrial and Nuclear DNA Markers Ann. Bot., May 1, 2006; 97(5): 793 - 805. [Abstract] [Full Text] [PDF]

				Z.-S. WANG, S.-Q. AN, H. LIU, X. LENG, J.-W. ZHENG, and Y.-H. LIU Genetic Structure of the Endangered Plant Neolitsea sericea (Lauraceae) from the Zhoushan Archipelago Using RAPD Markers Ann. Bot., January 2, 2005; 95(2): 305 - 313. [Abstract] [Full Text] [PDF]