HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (57) Citing Articles via Google Scholar Google Scholar Articles by Hedrén, M. Articles by Chase, M. W. Search for Related Content PubMed Articles by Hedrén, M. Articles by Chase, M. W. Agricola Articles by Hedrén, M. Articles by Chase, M. W.

Amplified fragment length polymorphisms (AFLP) reveal details of polyploid evolution in Dactylorhiza (Orchidaceae)¹

²Department of Systematic Botany, Lund University, Östra Vallgatan18–20, SE-223 61 Lund, Sweden; ³Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3DS, UK

The utility of the PCR-based AFLP technique (polymerase chain reaction; amplified fragment length polymorphisms) was explored in elucidating details of polyploid evolution in the Eurasian orchid genus Dactylorhiza. We emphasized Swedish taxa but also included some material from the British Isles and elsewhere in Europe. Three different sets of primers, amplifying different subsets of restriction fragments, independently revealed similar patterns for relationships among the Dactylorhiza samples investigated. The AFLP data support the general picture of polyploid evolution in Dactylorhiza, i.e., that allotetraploid derivatives have arisen repeatedly as a result of hybridization beween the two parental groups D. incarnata s.l. (sensu lato; diploid marsh orchids) and the D. maculata group (spotted orchids). Within the incarnata s.l. group, morphologically defined varieties were interdigitated. The D. maculata group consisted of two distinct subgroups, one containing autotetraploid D. maculata subsp. maculata and the other containing diploid D. maculata subsp. fuchsii. Allotetraploids showed a high degree of additivity for the putative parental genomes, and relationships among them were partly correlated to morphologically based entities, but also to geographic distribution. Thus, allotetraploid taxa from the British Isles clustered together, rather than with morphologically similar plants from other areas.

Key Words: AFLP • Dactylorhiza • Orchidaceae • phylogeny • polyploid evolution • systematics

This article has been cited by other articles:

				M. Hedren, S. Nordstrom, H. A. P. Hovmalm, H. A. Pedersen, and S. Hansson Patterns of polyploid evolution in Greek marsh orchids (Dactylorhiza; Orchidaceae) as revealed by allozymes, AFLPs, and plastid DNA data Am. J. Botany, July 1, 2007; 94(7): 1205 - 1218. [Abstract] [Full Text] [PDF]

				R. M. BATEMAN and P. J. RUDALL Evolutionary and Morphometric Implications of Morphological Variation Among Flowers Within an Inflorescence: A Case-Study Using European Orchids Ann. Bot., November 1, 2006; 98(5): 975 - 993. [Abstract] [Full Text] [PDF]

				E. PEREZ-COLLAZOS and P. CATALAN Palaeopolyploidy, Spatial Structure and Conservation Genetics of the Narrow Steppe Plant Vella pseudocytisus subsp. paui (Vellinae, Cruciferae) Ann. Bot., April 1, 2006; 97(4): 635 - 647. [Abstract] [Full Text] [PDF]

				M. F. FAY, R. S. COWAN, and I. J. LEITCH The Effects of Nuclear DNA Content (C-value) on the Quality and Utility of AFLP Fingerprints Ann. Bot., January 1, 2005; 95(1): 237 - 246. [Abstract] [Full Text] [PDF]

				G. Pellegrino, A. Musacchio, M. E. Noce, A. M. Palermo, and A. Widmer Reproductive Versus Floral Isolation Among Morphologically Similar Serapias L. Species (Orchidaceae) J. Hered., January 1, 2005; 96(1): 15 - 23. [Abstract] [Full Text] [PDF]

				E. A. Kellogg and J. L. Bennetzen The evolution of nuclear genome structure in seed plants Am. J. Botany, October 1, 2004; 91(10): 1709 - 1725. [Abstract] [Full Text] [PDF]

				A. B. Shipunov, M. F. Fay, Y. Pillon, R. M. Bateman, and M. W. Chase Dactylorhiza (Orchidaceae) in European Russia: combined molecular and morphological analysis Am. J. Botany, September 1, 2004; 91(9): 1419 - 1426. [Abstract] [Full Text] [PDF]

				D. H. Goldman, R. K. Jansen, C. van den Berg, I. J. Leitch, M. F. Fay, and M. W. Chase Molecular and cytological examination of Calopogon (Orchidaceae, Epidendroideae): circumscription, phylogeny, polyploidy, and possible hybrid speciation Am. J. Botany, May 1, 2004; 91(5): 707 - 723. [Abstract] [Full Text] [PDF]

				V. Gobert, S. Moja, M. Colson, and P. Taberlet Hybridization in the section Mentha (Lamiaceae) inferred from AFLP markers Am. J. Botany, December 1, 2002; 89(12): 2017 - 2023. [Abstract] [Full Text] [PDF]