HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Szalanski, A. L. Articles by Petersen, J. Search for Related Content PubMed PubMed Citation Articles by Szalanski, A. L. Articles by Petersen, J. Agricola Articles by Szalanski, A. L. Articles by Petersen, J.

Origin and conservation genetics of the threatened Ute ladies'-tresses, Spiranthes diluvialis (Orchidaceae)¹

¹ Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, Nebraska 68583 USA; ³ Nebraska Game and Parks Commission, Lincoln, Nebraska 68503 USA

The Ute ladies'-tresses, Spiranthes diluvialis, is listed as a threatened orchid in west-central United States by the Federal government. Information on its origin and patterns of genetic variation is needed to develop effective conservation strategies for this species. DNA sequencing and polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) was used to evaluate genetic variation and structure of 23 populations of S. diluvialis. In addition, four congeneric species were analyzed to determine possible origins of the putative allotetraploid S. diluvialis. DNA sequencing and PCR-RFLP analysis of the nuclear ribosomal internal transcribed spacer (ITS) and mitochondrial and chloroplast DNA noncoding regions revealed no genetic variation within or among populations of S. diluvialis. DNA sequencing revealed that S. diluvialis has rDNA of both S. magnicamporum and S. romanzoffiana, supporting the proposed origin of the allotetraploid. Parsimony and maximum likelihood analyses of cpDNA and mtDNA sequences revealed that these S. diluvialis organellar sequences were most closely related to those of S. romanzoffiana, providing evidence that the latter species is the maternal parent of S. diluvialis. The lack of genetic diversity is significant for the development of a long-term conservation strategy for S. diluvialis.

Key Words: phylogenetics • population genetics • Orchidaceae • Spiranthes • threatened species

This article has been cited by other articles:

				I. Avila-Diaz and K. Oyama Conservation genetics of an endemic and endangered epiphytic Laelia speciosa (Orchidaceae) Am. J. Botany, February 1, 2007; 94(2): 184 - 193. [Abstract] [Full Text] [PDF]

				M. Sun and K. C. Wong Genetic structure of three orchid species with contrasting breeding systems using RAPD and allozyme markers Am. J. Botany, December 1, 2001; 88(12): 2180 - 2188. [Abstract] [Full Text] [PDF]