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Galls on the marine red alga Prionitislanceolata (Halymeniaceae): specific induction and subsequentdevelopment of an algal–bacterial symbiosis¹

^a Department ofBiology, University of California, Santa Cruz 1156 High Street, SantaCruz, CA 95064

Gall formation in Prionitis lanceolata is associated with aspecific eubacterium (Proteobacteria [alphasubclass], Rhodobacter grouping), which, typical ofbacterial symbionts, has not yet been cultivated or isolated in pureculture. This investigation tested the hypothesis that P.lanceolata gall formation was caused by the associated eubacteriumusing a species-specific rDNA probe (S-S-P.l.sym-0949-a-A-25) toidentify and assay for symbiont presence during consecutive laboratoryinduction trials. Gall induction was quantified and whole-cell in situhybridization used to determine the relative percentage of symbioticeubacteria in inoculation homogenates. In situ hybridization ofsymbionts in sections allowed localization and monitoring of thismicrobe during gall development. Induction trial results indicate asignificant correlation between bacterial symbiont presence and gallinitiation (P = 0.00005). The gall bacterium comprisedthe majority of the eubacteria hybridized in laboratory inductionhomogenates (85–97%), in galls induced in the laboratoryand in three algal populations in nature. The evidence presented heredemonstrates the causative role of the identified eubacterium in gallinduction and formation. This investigation is significant in theapplication of molecular methods towards understanding the roles ofnoncultivable marine bacteria in marine algal–microbeinteractions.

Key Words: eubacteria • galls • Halymeniaceae • insitu hybridization • Prionitislanceolata • redalgae • symbiosis

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