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American Journal of Botany, Vol 84, 1658, Copyright © 1997 by Botanical Society of America, Inc.

GENETICS AND MOLECULAR BIOLOGY

Conservation of microsatellites among tropical trees (Leguminosae)

S Dayanandan, KS Bawa and R Kesseli

Although microsatellites or simple sequence repeats (SSRs) have become a popular tool in genetic mapping and gene flow studies, their utility is limited due to paucity of information about DNA sequences in plants. We tested the utility of microsatellite markers characterized for the tropical tree Pithecellobium elegans as a genetic tool for related species. The results indicate that SSR loci are conserved among closely related species, and SSR primers developed for P. elegans could be successfully used as a genetic tool in several species of the tribe Ingeae. This study indicates that there is high potential for the transfer of SSR markers among closely related taxa, circumventing laborious cloning and screening procedures involved in characterizing SSR loci for many species.


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