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American Journal of Botany, Vol 84, 79, Copyright © 1997 by Botanical Society of America, Inc.

SYSTEMATICS

Presence of alpha-amylase inhibitor in some members of the subtribe phaseolinae (phaseoleae: fabaceae)

JJ Pueyo and A Delgado-Salinas

The presence of alpha-amylase inhibitor (alpha-AI), phytohemagglutinin (PHA), and phaseolin was determined by immunochemical and biochemical methods in different members of the subtribe Phaseolinae (Phaseoleae: Fabaceae). Active alpha-AI was present in taxa from the Phaseolus vulgaris-P. coccineus complex (P. vulgaris, P. coccineus, and P. polyanthus). as well as in P. acutifolius in accordance with previous molecular data separating these groups of species from others in the genus. All other Phaseolus species tested lacked alpha-amylase inhibitory activity, although some of them had immunoreactive polypeptides. Alpha-AI was found to be a polymorphic trait among wild and cultivated accessions of P. vulgaris, P. coccineus, and P. acutifolius. The presence of alpha-Al is not exclusive of the genus Phaseolus as one of the Vigna species sampled, V. linearis, also contained alpha-amylase inhibitory activity.




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Copyright © 1997 by the Botanical Society of America, Inc.